• ホーム
  • 研究内容
  • メンバー
  • 研究室・機器紹介
  • 活動報告
  • 受賞・プレス
  • アルバム

活動報告

論文業績

2020 Publication

Original Articles

  1. S. Ito, M. Oishi, S. Ogata, T. Uemura, P.O. Couraud, T. Masuda, S. Ohtsuki: Identification of Cell-Surface Proteins Endocytosed by Human Brain Microvascular Endothelial Cells In Vitro. Pharmaceutics. 12 (2020)
  2. M. Nambu, S. Nishiumi, T. Kobayashi, T. Masuda, S. Ito, M. Yoshida, S. Ohtsuki: Effects of differences in pre-analytical processing on blood protein profiles determined with SWATH-MS. J Proteomics. 223:103824 (2020)
  3. Y. Yamashiro, B.Q. Thang, K. Ramirez, S.J. Shin, T. Kohata, S. Ohata, T.A.V. Nguyen, S. Ohtsuki, K. Nagayama, H. Yanagisawa: Matrix mechanotransduction mediated by thrombospondin-1/integrin/YAP in the vascular remodeling. Proc Natl Acad Sci U S A. 117:9896-9905 (2020)
  4. J. Zhang, T. Furuta, H. Sabit, S. Tamai, S. Jiapaer, Y. Dong, M. Kinoshita, Y. Uchida, S. Ohtsuki, T. Terasaki, S. Zhao, M. Nakada: Gelsolin inhibits malignant phenotype of glioblastoma and is regulated by miR-654-5p and miR-450b-5p. Cancer Sci.  (2020)
  5. C. Liu, Y. Kobashigawa, S. Yamauchi, N. Fukuda, T. Sato, T. Masuda, S. Ohtsuki, H. Morioka: Convenient method of producing cyclic single-chain Fv antibodies by split-intein-mediated protein ligation and chaperone co-expression. J Biochem.  (2020)
  6. S. Yamaguchi, S. Ito, T. Masuda, P.O. Couraud, S. Ohtsuki: Novel cyclic peptides facilitating transcellular blood-brain barrier transport of macromolecules in vitro and in vivo. J Control Release. 321:744-755 (2020)
  7. T. Uemura, S. Ito, T. Masuda, H. Shimbo, T. Goto, H. Osaka, T. Wada, P.O. Couraud, S. Ohtsuki: Cyclocreatine Transport by SLC6A8, the Creatine Transporter, in HEK293 Cells, a Human Blood-Brain Barrier Model Cell, and CCDSs Patient-Derived Fibroblasts. Pharm Res. 37:61 (2020)
  8. M. Tachikawa, Y. Kaneko, S. Ohtsuki, Y. Uchida, M. Watanabe, H. Ohtsuka, T. Terasaki: Targeted Proteomics-Based Quantitative Protein Atlas of Pannexin and Connexin Subtypes in Mouse and Human Tissues and Cancer Cell Lines.  J Pharm Sci. 109:1161-1168 (2020)
  9. T. Masuda, N. Sugiyama, M. Tomita, S. Ohtsuki, Y. Ishihama: Mass Spectrometry-Compatible Subcellular Fractionation for Proteomics.  J Proteome Res. 19:75-84 (2020)

2019 Publications

Original Articles

  1. N. Tateshita, N. Miura, H. Tanaka, T. Masuda, S. Ohtsuki, K. Tange, Y. Nakai, H. Yoshioka, H. Akita: Development of a lipoplex-type mRNA carrier composed of an ionizable lipid with a vitamin E scaffold and the KALA peptide for use as an ex vivo dendritic cell-based cancer vaccine. Journal of Controlled Release. 30:36-46 (2019)
  2. S. Ogata, S. Ito, T. Masuda, S. Ohtsuki: Changes of Blood-Brain Barrier and Brain Parenchymal Protein Expression Levels of Mice under Different Insulin-Resistance Conditions Induced by High-Fat Diet. Pharmaceutical research. 36:141 (2019)
  3. M. Nambu, T. Masuda, S. Ito, K. Kato, T. Kojima, H. Daiko, Y. Ito, K. Honda, S. Ohtsuki: Leucine-rich alpha-2-glycoprotein 1 in serum is a possible biomarker to predict response to preoperative chemoradiotherapy for esophageal cancer. Biol Pharm Bull. 42:1766-1771 (2019)
  4. E. Takeo, Y. Sugiura, T. Uemura, K. Nishimoto, M. Yasuda, E. Sugiyama, S. Ohtsuki, T. Higashi, T. Nishikawa, M. Suematsu, E. Fukusaki, S. Shimma: Tandem Mass Spectrometry Imaging Reveals Distinct Accumulation Patterns of Steroid Structural Isomers in Human Adrenal Glands.  Anal Chem.  91:8918-8925 (2019)
  5. T. Masuda, T. Hoshiyama, T. Uemura, M. Hirayama-Kurogi, S. Ogata, A. Furukawa, P.O. Couraud, T. Furihata, S. Ito, S. Ohtsuki: Large-Scale Quantitative Comparison of Plasma Transmembrane Proteins between Two Human Blood-Brain Barrier Model Cell Lines, hCMEC/D3 and HBMEC/cibeta.  Mol Pharm.  16:2162-2171 (2019)
  6. T. Kuno, M. Hirayama-Kurogi, S. Ito, S. Ohtsuki: Proteomic analysis of small intestinal epithelial cells in antibiotic-treated mice: Changes in drug transporters and metabolizing enzymes.  Drug Metab Pharmacokinet.  34:159-162 (2019)
  7. S. Ito, G. Honda, Y. Fujino, S. Ogata, M. Hirayama-Kurogi, S. Ohtsuki: Knockdown of Orphan Transporter SLC22A18 Impairs Lipid Metabolism and Increases Invasiveness of HepG2 Cells.  Pharm Res.  36:39 (2019)
  8. Y. Hoshi, Y. Uchida, M. Tachikawa, S. Ohtsuki, P.O. Couraud, T. Suzuki, T. Terasaki: Oxidative stress-induced activation of Abl and Src kinases rapidly induces P-glycoprotein internalization via phosphorylation of caveolin-1 on tyrosine-14, decreasing cortisol efflux at the blood-brain barrier.  J Cereb Blood Flow Metab.  271678X18822801 (2019)
  9. T. Kimura, A. Tsukada, T. Fukutomi, K. Ichida, S. Ohtsuki, H. Sakurai: Urate Transport via Paracellular Route across Epithelial Cells.  Biol Pharm Bull.  42:43-49 (2019)
  10. D. Kobayashi, T. Tokuda, K. Sato, H. Okanishi, M. Nagayama, M. Hirayama-Kurogi, S. Ohtsuki, N. Araki: Identification of a Specific Translational Machinery via TCTP-EF1A2 Interaction Regulating NF1-associated Tumor Growth by Affinity Purification and Data-independent Mass Spectrometry Acquisition (AP-DIA).  Mol Cell Proteomics.  18:245-262 (2019)
  11. S. Nishiumi, T. Kohata, T. Kobayashi, Y. Kodama, S. Ohtsuki, M. Yoshida: Comparison of venous and fingertip plasma using non-targeted proteomics and metabolomics.  Talanta.  192:182-188 (2019)

著書

    1. 米山敏広、大槻純男：第6章 早期膵がんの血中バイオマーカーへの新しいアプローチ、「早期発見・予防にむけた次世代がん検査技術の最前線」、シーエムシー出版、54-62 (2019)

2018 Pubilcation

Original Articles

    1. T. Akazawa, Y. Uchida, E. Miyauchi, M. Tachikawa, S. Ohtsuki, T. Terasaki: High Expression of UGT1A1/1A6 in Monkey Small Intestine: Comparison of Protein Expression Levels of Cytochromes P450, UDP-Glucuronosyltransferases, and Transporters in Small Intestine of Cynomolgus Monkey and Human.  Mol Pharm.  15:127-140 (2018)
    2. T. Kuno, M. Hirayama-Kurogi, S. Ito, S. Ohtsuki: Reduction in hepatic secondary bile acids caused by short-term antibiotic-induced dysbiosis decreases mouse serum glucose and triglyceride levels.  Sci Rep.  8:1253 (2018)
    3. K. Sasaki, M. Tachikawa, Y. Uchida, S. Hirano, F. Kadowaki, M. Watanabe, S. Ohtsuki, T. Terasaki: ATP-Binding Cassette Transporter A Subfamily 8 Is a Sinusoidal Efflux Transporter for Cholesterol and Taurocholate in Mouse and Human Liver.  Mol Pharm.  15:343-355 (2018)
    4. E. Miyauchi, T. Furuta, S. Ohtsuki, M. Tachikawa, Y. Uchida, H. Sabit, W. Obuchi, T. Baba, M. Watanabe, T. Terasaki, M. Nakada: Identification of blood biomarkers in glioblastoma by SWATH mass spectrometry and quantitative targeted absolute proteomics.  PLoS One.  13:e0193799 (2018)
    5. S. Nakamura, S.I. Muramatsu, N. Takino, M. Ito, E.F. Jimbo, K. Shimazaki, T. Onaka, S. Ohtsuki, T. Terasaki, T. Yamagata, H. Osaka: Gene therapy for Glut1-deficient mouse using an adeno-associated virus vector with the human intrinsic GLUT1 promoter.  J Gene Med.  20:e3013 (2018)
    6. Y. Yamasaki, K. Kobayashi, F. Okuya, N. Kajitani, K. Kazuki, S. Abe, S. Takehara, S. Ito, S. Ogata, T. Uemura, S. Ohtsuki, G. Minegishi, H. Akita, K. Chiba, M. Oshimura, Y. Kazuki: Characterization of P-Glycoprotein Humanized Mice Generated by Chromosome Engineering Technology: Its Utility for Prediction of Drug Distribution to the Brain in Humans.  Drug Metab Dispos.  46:1756-1766 (2018)
    7. M. Fukuda, T. Yoshizawa, M.F. Karim, S.U. Sobuz, W. Korogi, D. Kobayasi, H. Okanishi, M. Tasaki, K. Ono, T. Sawa, Y. Sato, M. Chirifu, T. Masuda, T. Nakamura, H. Tanoue, K. Nakashima, Y. Kobashigawa, H. Morioka, E. Bober, S. Ohtsuki, Y. Yamagata, Y. Ando, Y. Oike, N. Araki, S. Takeda, H. Mizuta, K. Yamagata: SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix.  Nat Commun.  9:2833 (2018)
    8. T. Yamauchi, T. Masuda, MC. Canver, M. Seiler, Y. Semba, M. Shboul, M. Al-Raqad, M. Maeda, VA. C. Schoonenberg, MA. Cole, C. M. Trevino, Y. Ishikawa, Q. Yao, M. Nakano, F. Arai, SH. Orkin, B. Reversade, S. Buonamici, L. Pinello, K. Akashi, DE. Bauer, T. Maeda: Genome-wide CRISPR-Cas9 screen identifies leukemia-specific dependence on a pre-mRNA metabolic pathway regulated by the DCPS decapping enzyme. Cancer Cell, DOI10.1016/j.ccell.2018.01.012 (2018)
    9. Y. Uchida, T. Sumiya, M. Tachikawa, T. Yamakawa, S. Murata, Y. Yagi, K. Sato, A. Stephan, K. Ito, S. Ohtsuki, P.O. Couraud, T. Suzuki, T. Terasaki: Involvement of Claudin-11 in Disruption of Blood-Brain, -Spinal Cord, and -Arachnoid Barriers in Multiple Sclerosis.  Mol Neurobiol.  (2018)
    10. S. Ito, Y. Fujino, S. Ogata, M. Hirayama-Kurogi, S. Ohtsuki: Involvement of an Orphan Transporter, SLC22A18, in Cell Growth and Drug Resistance of Human Breast Cancer MCF7 Cells.  J Pharm Sci.  107:3163-3170 (2018)
    11. H. Maruyama, T. Kimura, H. Liu, S. Ohtsuki, Y. Miyake, M. Isogai, F. Arai, A. Honda: Influenza virus replication raises the temperature of cells.  Virus Res.  257:94-101 (2018)

総説

    1. 米山敏広、大槻純男：早期膵がんの血中バイオマーカーへの新しいアプローチ、月刊ファインケミカル、47:39-45 (2018)
    2. 伊藤慎悟：生体関門輸送機能を基盤とした疾患メカニズムの解明と治療への展開 YAKUGAKU ZASSHI (Accepted)

解説

    1. 山口駿介、伊藤慎悟: Identification of cyclic peptides for facilitation of transcellular transport of phages across intestinal epithelium in vitro and in vivo、Drug Delivery System、日本DDS学会、33-1、 56 (2018)

総合論文

    1. 伊藤慎悟、大槻純男：SWATH法を用いた網羅的定量プロテオーム解析による薬物動態研究の新展開 Proteome Letters (Accepted)

トピックス

    1. 伊藤慎悟：腸内フローラが薬の効果と副作用に与える影響、バイオサイエンスとインダストリー(B&I)、76 (5) (2018)

著書

    1. 久野琢矢、大槻純男：第3章、第9節 腸内細菌叢の変化が薬効に与える影響、「腸内細菌叢を標的にしたい薬品と保健機能食品の開発」、技術情報協会、164-70 (2018)

2017 Publications

Original Articles

  1. K. Shirotani, K. Matsuo, S. Ohtsuki, T. Masuda, M. Asai, Y. Kutoku, Y. Ohsawa, Y. Sunada, T. Kondo, H. Inoue, N. Iwata: A simplified and sensitive method to identify Alzheimer’s disease biomarker candidates using patient-derived induced pluripotent stem cells (iPSCs).  J Biochem. 162:391-394 (2017)
  2. H. Nagahori, K. Nakamura, K. Sumida, S. Ito, S. Ohtsuki: Combining genomics to identify the pathways of post-transcriptional non-genotoxic signaling and energy homeostasis in livers of rats treated with the PXR agonist, PCN.  J Proteome Res. 16:3634-3645 (2017)
  3. B.C. Collins, C.L. Hunter, Y. Liu, B. Schilling, G. Rosenberger, S.L. Bader, D.W. Chan, B.W. Gibson, A.C. Gingras, J.M. Held, M. Hirayama-Kurogi, G. Hou, C. Krisp, B. Larsen, L. Lin, S. Liu, M.P. Molloy, R.L. Moritz, S. Ohtsuki, R. Schlapbach, N. Selevsek, S.N. Thomas, S.C. Tzeng, H. Zhang, R. Aebersold: Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry.  Nat Commun.  8:291 (2017)
  4. S. Yamaguchi, S. Ito, M. Kurogi-Hirayama, S. Ohtsuki: Identification of cyclic peptides for facilitation of transcellular transport of phages across intestinal epithelium in vitro and in vivo.  J Control Release.  262:232-238 (2017)
  5. S. Ito, M. Yanai, S. Yamaguchi, P.O. Couraud, S. Ohtsuki: Regulation of Tight-Junction Integrity by Insulin in an In Vitro Model of Human Blood-Brain Barrier.  J Pharm Sci.  106:2599-2605 (2017)
  6. M. Hirayama-Kurogi, Y. Takizawa, Y. Kunii, J. Matsumoto, A. Wada, M. Hino, H. Akatsu, Y. Hashizume, S. Yamamoto, T. Kondo, S. Ito, M. Tachikawa, S.I. Niwa, H. Yabe, T. Terasaki, M. Setou, S. Ohtsuki: Downregulation of GNA13-ERK network in prefrontal cortex of schizophrenia brain identified by combined focused and targeted quantitative proteomics.  J Proteomics.  158:31-42 (2017)
  7. T. Yoneyama, S. Ohtsuki, M. Tachikawa, Y. Uchida, T. Terasaki: Scrambled Internal Standard (sIS) Method for High-Throughput Protein Quantification by MALDI-MS/MS.  J Proteome Res. 16:1556-1565 (2017)
  8. Y. Hoshi, Y. Uchida, M. Tachikawa, S. Ohtsuki, T. Terasaki: Actin filament-associated protein 1 (AFAP-1) is a key mediator in inflammatory signaling-induced rapid attenuation of intrinsic P-gp function in human brain capillary endothelial cells.  Journal of Neurochemistry.  141:247-262 (2017)
  9. T. Uemura, S. Ito, Y. Ohta, M. Tachikawa, T. Wada, T. Terasaki, S. Ohtsuki: Abnormal N-Glycosylation of a Novel Missense Creatine Transporter Mutant, G561R, Associated with Cerebral Creatine Deficiency Syndromes Alters Transporter Activity and Localization.  Biol Pharm Bull.  40:49-55 (2017)
  10. H. Kuroda, M. Tachikawa, Y. Uchida, K. Inoue, H. Ohtsuka, S. Ohtsuki, M. Unno, T. Terasaki: All-trans retinoic acid enhances gemcitabine cytotoxicity in human pancreatic cancer cell line AsPC-1 by up-regulating protein expression of deoxycytidine kinase.  Eur J Pharm Sci.  103:116-121 (2017)
  11. T. Miura, M. Tachikawa, H. Ohtsuka, K. Fukase, S. Nakayama, N. Sakata, F. Motoi, T. Naitoh, Y. Katayose, Y. Uchida, S. Ohtsuki, T. Terasaki, M. Unno: Application of Quantitative Targeted Absolute Proteomics to Profile Protein Expression Changes of Hepatic Transporters and Metabolizing Enzymes During Cholic Acid-Promoted Liver Regeneration.  J Pharm Sci.  106:2499-2508 (2017)
  12. S. Nakamura, H. Osaka, S.I. Muramatsu, N. Takino, M. Ito, S. Aoki, E.F. Jimbo, K. Shimazaki, T. Onaka, S. Ohtsuki, T. Terasaki, T. Yamagata: Gene therapy for a mouse model of glucose transporter-1 deficiency syndrome.  Mol Genet Metab Rep.  10:67-74 (2017)
  13. Y. Ochiai, Y. Uchida, S. Ohtsuki, M. Tachikawa, S. Aizawa, T. Terasaki: The blood-brain barrier fatty acid transport protein 1 (FATP1/SLC27A1) supplies docosahexaenoic acid to the brain, and insulin facilitates transport.  J Neurochem.  141:400-412 (2017)
総説
  1. 増田豪、緒方星陵、高畑智宏、伊藤慎悟、大槻純男：定量プロテオミクスの薬物治療、診断への展開、臨床化学、46:283-290 (2017)

2016 Publications

Original Articles
  1. T. Yoneyama, S. Ohtsuki, K. Honda, M. Kobayashi, M. Iwasaki, Y. Uchida, T. Okusaka, S. Nakamori, M. Shimahara, T. Ueno, A. Tsuchida, N. Sata, T. Ioka, Y. Yasunami, T. Kosuge, T. Kaneda, T. Kato, K. Yagihara, S. Fujita, W. Huang, T. Yamada, M. Tachikawa, T. Terasaki: Identification of IGFBP2 and IGFBP3 As Compensatory Biomarkers for CA19-9 in Early-Stage Pancreatic Cancer Using a Combination of Antibody-Based and LC-MS/MS-Based Proteomics. PLoS One. 11:e0161009 (2016)
  2. T. Kuno, M. Hirayama-Kurogi, S. Ito, S. Ohtsuki: Effect of Intestinal Flora on Protein Expression of Drug-Metabolizing Enzymes and Transporters in the Liver and Kidney of Germ-Free and Antibiotics-Treated Mice. Mol Pharm. 13:2691-701 (2016)
  3. T. Akazawa, Y. Uchida, M. Tachikawa, S. Ohtsuki, T. Terasaki: Quantitative Targeted Absolute Proteomics of Transporters and Pharmacoproteomics-Based Reconstruction of P-Glycoprotein Function in Mouse Small Intestine. Mol Pharm. 13:2443-56 (2016)
  4. K. Nakamura, M. Hirayama-Kurogi, S. Ito, T. Kuno, T. Yoneyama, W. Obuchi, T. Terasaki, S. Ohtsuki: Large-scale multiplex absolute protein quantification of drug-metabolizing enzymes and transporters in human intestine, liver and kidney microsomes by SWATH-MS: Comparison with MRM/SRM and HR-MRM/PRM. Proteomics. 16:2106-17 (2016)
  5. Y. Uchida, T. Toyohara, S. Ohtsuki, Y. Moriyama, T. Abe, T. Terasaki: Quantitative Targeted Absolute Proteomics for 28 Transporters in Brush-Border and Basolateral Membrane Fractions of Rat Kidney. J Pharm Sci. 105:1011-6 (2016)
  6. S Ito, M Ménard, T Atkinson, L Brown, J Whitfield, B Chakravarthy: Relative expression of the p75 neurotrophin receptor, tyrosine receptor kinase A, and insulin receptor in SH-SY5Y neuroblastoma cells and hippocampi from Alzheimer’s disease patients. Neurochem Int.101:22-29 (2016)
著書
  1. 大槻純男：第３章　細菌学総論・細菌の遺伝、「微生物学・感染症学（第2版）」、塩田澄子、黒田照夫編、化学同人、東京、55-67 (2016)

2015 Publications

Original Articles
  1. Y. Uchida, S. Ohtsuki, J. Kamiie, K. Ohmine, R. Iwase, T. Terasaki: Quantitative targeted absolute proteomics for 28 human transporters in plasma membrane of Caco-2 cell monolayer cultured for 2, 3, and 4 weeks. Drug Metab Pharmacokinet. 30:205-8 (2015)
  2. T. Oyama, E. Negishi, H. Onigahara, N. Kusano, Y. Miyoshi, M. Mita, M. Nakazono, S. Ohtsuki, A. Ojida, W. Lindner, K. Hamase: Design and synthesis of a novel pre-column derivatization reagent with a 6-methoxy-4-quinolone moiety for fluorescence and tandem mass spectrometric detection and its application to chiral amino acid analysis. J Pharm Biomed Anal. 116:71-9 (2015)
  3. Y. Uchida, K. Ito, S. Ohtsuki, Y. Kubo, T. Suzuki, T. Terasaki: Major involvement of Na(+) -dependent multivitamin transporter (SLC5A6/SMVT) in uptake of biotin and pantothenic acid by human brain capillary endothelial cells. J Neurochem. 134:97-112 (2015)
  4. K. Ohmine, K. Kawaguchi, S. Ohtsuki, F. Motoi, H. Ohtsuka, J. Kamiie, T. Abe, M. Unno, T. Terasaki: Quantitative Targeted Proteomics of Pancreatic Cancer: Deoxycytidine Kinase Protein Level Correlates to Progression-Free Survival of Patients Receiving Gemcitabine Treatment. Mol Pharm. 12:3282-91 (2015)
  5. Y. Uchida, T. Toyohara, S. Ohtsuki, Y. Moriyama, T. Abe, T. Terasaki: Quantitative Targeted Absolute Proteomics for 28 Transporters in Brush-Border and Basolateral Membrane Fractions of Rat Kidney. J Pharm Sci. 105:1011-6 (2016)
  6. Y. Kaneko, M. Tachikawa, R. Akaogi, K. Fujimoto, M. Ishibashi, Y. Uchida, P.O. Couraud, S. Ohtsuki, K. Hosoya, T. Terasaki: Contribution of pannexin 1 and connexin 43 hemichannels to extracellular calcium-dependent transport dynamics in human blood-brain barrier endothelial cells. J Pharmacol Exp Ther. 353:192-200 (2015)
  7. Y. Kubo, S. Ohtsuki, Y. Uchida, T. Terasaki: Quantitative Determination of Luminal and Abluminal Membrane Distributions of Transporters in Porcine Brain Capillaries by Plasma Membrane Fractionation and Quantitative Targeted Proteomics. J Pharm Sci. 104:3060-8 (2015)
  8. T. Nishina, J. Numata, K. Nishina, K. Yoshida-Tanaka, K. Nitta, W. Piao, R. Iwata, S. Ito, H. Kuwahara, T. Wada, H. Mizusawa, T. Yokota:Chimeric Antisense Oligonucleotide Conjugated to alpha-Tocopherol. Mol Ther Nucleic Acids.4:e220 (2015)

2014 Publications

Original Articles
  1. Y. Uchida, S. Ohtsuki, T. Terasaki:Pharmacoproteomics-based reconstruction of in vivo p-glycoprotein function at blood-brain barrier and brain distribution of substrate verapamil in pentylenetetrazole-kindled epilepsy, spontaneous epilepsy, and phenytoin treatment models.Drug Metab Dispos.42:1719-26 (2014)
  2. Y. Uchida, K. Wakayama, S. Ohtsuki, M. Chiba, T. Ohe, Y. Ishii, T. Terasaki: Blood-brain barrier pharmacoproteomics-based reconstruction of the in vivo brain distribution of p-glycoprotein substrates in cynomolgus monkeys. J Pharmacol Exp Ther. 350:578-88 (2014)
  3. S. Ohtsuki, H. Kawakami, T. Inoue, K. Nakamura, C. Tateno, Y. Katsukura, W. Obuchi, Y. Uchida, J. Kamiie, T. Horie, T. Terasaki: Validation of uPA/SCID Mouse with Humanized Liver as a Human Liver Model: Protein Quantification of Transporters, Cytochromes P450, and UDP-Glucuronosyltransferases by LC-MS/MS. Drug Metab Dispos.42:1039-43 (2014)
  4. S. Ito, S. Ohtsuki, S. Murata, Y. Katsukura, H. Suzuki, M. Funaki, M. Tachikawa, T. Terasaki: Involvement of insulin-degrading enzyme in insulin- and atrial natriuretic peptide-sensitive internalization of amyloid-beta peptide in mouse brain capillary endothelial cells. J Alzheimers Dis.38:185-200 (2014)
  5. B Chakravarthy, S Ito, T Atkinson, C Gaudet, M Ménard, L Brown, J Whitfield:Evidence that a synthetic amyloid-ß oligomer-binding peptide (ABP) targets amyloid-ß deposits in transgenic mouse brain and human Alzheimer’s disease brain. Biochem Biophys Res Commun. 445(3):656-60 (2014).
Review Articles
  1. S. Ohtsuki, M. Hirayama, S. Ito, Y. Uchida, M. Tachikawa, T. Terasaki: Quantitative targeted proteomics for understanding the blood-brain barrier: towards pharmacoproteomics. Expert Rev Proteomics.11:303-13 (2014)

2013 Publications

Original Articles
  1. S. Ito, K. Matsumiya, S. Ohtsuki, J. Kamiie, T. Terasaki:Contributions of degradation and brain-to-blood elimination across the blood-brain barrier to cerebral clearance of human amyloid-beta peptide(1-40) in mouse brain.J Cereb Blood Flow Metab.33:1770-7 (2013)
  2. Y. Uchida, M. Tachikawa, W. Obuchi, Y. Hoshi, Y. Tomioka, S. Ohtsuki, T. Terasaki:A study protocol for quantitative targeted absolute proteomics (QTAP) by LC-MS/MS: application for inter-strain differences in protein expression levels of transporters, receptors, claudin-5, and marker proteins at the blood-brain barrier in ddY, FVB, and C57BL/6J mice.Fluids Barriers CNS.10:21 (2013)
  3. Y. Hoshi, Y. Uchida, M. Tachikawa, T. Inoue, S. Ohtsuki, T. Terasaki:Quantitative atlas of blood-brain barrier transporters, receptors, and tight junction proteins in rats and common marmoset.J Pharm Sci.(2013)
  4. A. Sakamoto, T. Matsumaru, N. Yamamura, Y. Uchida, M. Tachikawa, S. Ohtsuki, T. Terasaki:Quantitative expression of human drug transporter proteins in lung tissues: Analysis of regional, gender, and interindividual differences by liquid chromatography-tandem mass spectrometry.J Pharm Sci.(2013)
  5. T. Yoneyama, S. Ohtsuki, M. Ono, K. Ohmine, Y. Uchida, T. Yamada, M. Tachikawa, T. Terasaki:Quantitative Targeted Absolute Proteomics-Based Large-Scale Quantification of Proline-Hydroxylated alpha-Fibrinogen in Plasma for Pancreatic Cancer Diagnosis.J Proteome Res.12:753-62 (2013)
  6. W. Obuchi, S. Ohtsuki, Y. Uchida, K. Ohmine, T. Yamori, T. Terasaki:Identification of transporters associated with Etoposide sensitivity of stomach cancer cell lines and methotrexate sensitivity of breast cancer cell lines by quantitative targeted absolute proteomics.Mol Pharmacol.83:490-500 (2013)
  7. Y. Kazuki, K. Kobayashi, S. Aueviriyavit, T. Oshima, Y. Kuroiwa, Y. Tsukazaki, N. Senda, H. Kawakami, S. Ohtsuki, S. Abe, M. Takiguchi, H. Hoshiya, N. Kajitani, S. Takehara, K. Kubo, T. Terasaki, K. Chiba, K. Tomizuka, M. Oshimura:Trans-chromosomic mice containing a human CYP3A cluster for prediction of xenobiotic metabolism in humans.Hum Mol Genet.22:578-92 (2013)
  8. S. Ohtsuki, C. Ikeda, Y. Uchida, Y. Sakamoto, F. Miller, F. Glacial, X. Decleves, J.M. Scherrmann, P.O. Couraud, Y. Kubo, M. Tachikawa, T. Terasaki:Quantitative targeted absolute proteomic analysis of transporters, receptors and junction proteins for validation of human cerebral microvascular endothelial cell line hCMEC/D3 as a human blood-brain barrier model.Mol Pharm.10:289-96 (2013)
総説
  1. 伊藤慎悟、大槻純男、寺崎哲也、脳内アミロイドβクリアランスにおける血液脳関門排出輸送分子機構とその破綻。細胞工学 vol.32 No.9　2013年9月号

2012 Publications

Original Articles
  1. K. Ohmine, K. Kawaguchi, S. Ohtsuki, F. Motoi, S. Egawa, M. Unno, T. Terasaki:Attenuation of phosphorylation by deoxycytidine kinase is key to acquired gemcitabine resistance in a pancreatic cancer cell line: targeted proteomic and metabolomic analyses in PK9 cells.Pharm Res.29:2006-16 (2012)
  2. A. Yoshikawa, M. Nakada, S. Ohtsuki, Y. Hayashi, W. Obuchi, Y. Sato, C. Ikeda, T. Watanabe, Y. Kawahara, T. Hasegawa, H. Sabit, D. Kita, Y. Hayashi, Y. Nakanuma, T. Terasaki, J.I. Hamada:Recurrent anaplastic meningioma treated by sunitinib based on results from quantitative proteomics.Neuropathol Appl Neurobiol.38:105-10 (2012)
  3. S. Ohtsuki, O. Schaefer, H. Kawakami, T. Inoue, S. Liehner, A. Saito, N. Ishiguro, W. Kishimoto, E. Ludwig-Schwellinger, T. Ebner, T. Terasaki:Simultaneous Absolute Protein Quantification of Transporters, Cytochromes P450, and UDP-Glucuronosyltransferases as a Novel Approach for the Characterization of Individual Human Liver: Comparison with mRNA Levels and Activities.Drug Metab Dispos.40:83-92 (2012)
  4. O. Schaefer, S. Ohtsuki, H. Kawakami, T. Inoue, S. Liehner, A. Saito, A. Sakamoto, N. Ishiguro, T. Matsumaru, T. Terasaki, T. Ebner:Absolute Quantification and Differential Expression of Drug Transporters, Cytochrome P450 Enzymes, and UDP-Glucuronosyltransferases in Cultured Primary Human Hepatocytes.Drug Metab Dispos.40:93-103 (2012)
  5. B. Chakravarthy, C. Gaudet, M. Menard, L. Brown, T. Atkinson, F.M. Laferla, S. Ito, U. Armato, I. Dal Pra, J. Whitfield:Reduction of the immunostainable length of the hippocampal dentate granule cells’ primary cilia in 3xAD-transgenic mice producing human Abeta(1-42) and tau.Biochem Biophys Res Commun.427:218-22 (2012)
  6. S. Ito, M. Menard, T. Atkinson, C. Gaudet, L. Brown, J. Whitfield, B. Chakravarthy:Involvement of insulin-like growth factor 1 receptor signaling in the amyloid-beta peptide oligomers-induced p75 neurotrophin receptor protein expression in mouse hippocampus.J Alzheimers Dis.31:493-506 (2012)
  7. B. Chakravarthy, M. Menard, S. Ito, C. Gaudet, I. Dal Pra, U. Armato, J. Whitfield:Hippocampal membrane-associated p75NTR levels are increased in Alzheimer’s disease.J Alzheimers Dis.30:675-84 (2012)
総説
  1. 大槻純男：抗体に依存しない新しいタンパク質定量技術の個別化治療への応用、実験医学、30:1046-1056 (2012)
  2. 大槻純男：定量的標的プロテオミクスによるファーマコプロテオミクスアプローチ、薬学雑誌、132:479-487 (2012)
  3. 大槻純男：定量的標的プロと恵美楠による生化学の新展開：抗体を用いない汎用的タンパク質定量技術、生化学、84:911-919, 2012

2011 Publications

Original Articles
  1. S. Akanuma, Y. Uchida, S. Ohtsuki, M. Tachikawa, T. Terasaki, K. Hosoya:Attenuation of prostaglandin E2 elimination across the mouse blood-brain barrier in lipopolysaccharide-induced inflammation and additive inhibitory effect of cefmetazole.Fluids Barriers CNS.8:24 (2011)
  2. Y. Uchida, S. Ohtsuki, Y. Katsukura, C. Ikeda, T. Suzuki, J. Kamiie, T. Terasaki:Quantitative targeted absolute proteomics of human blood-brain barrier transporters and receptors.J Neurochem.117:333-45 (2011)
  3. M. Fujiyoshi, M. Tachikawa, S. Ohtsuki, S. Ito, Y. Uchida, S. Akanuma, J. Kamiie, T. Hashimoto, K. Hosoya, T. Iwatsubo, T. Terasaki:Amyloid-beta peptide(1-40) elimination from cerebrospinal fluid involves low-density lipoprotein receptor-related protein 1 at the blood-cerebrospinal fluid barrier.J Neurochem.118:407-15 (2011)
  4. R. Shawahna, Y. Uchida, X. Decleves, S. Ohtsuki, S. Yousif, S. Dauchy, A. Jacob, F. Chassoux, C. Daumas-Duport, P.O. Couraud, T. Terasaki, J.M. Scherrmann:Transcriptomic and Quantitative Proteomic Analysis of Transporters and Drug Metabolizing Enzymes in Freshly Isolated Human Brain Microvessels.Mol Pharm.8:1332-1341 (2011)
  5. Y. Uchida, S. Ohtsuki, J. Kamiie, T. Terasaki:Blood-Brain Barrier (BBB) Pharmacoproteomics (PPx): Reconstruction of In Vivo Brain Distribution of 11 P-glycoprotein Substrates based on the BBB Transporter Protein Concentration, In Vitro Intrinsic Transport Activity, and Unbound Fraction in Plasma and Brain in Mice.J Pharmacol Exp Ther.339:579-88 (2011)
  6. S. Akanuma, Y. Uchida, S. Ohtsuki, J. Kamiie, M. Tachikawa, T. Terasaki, K. Hosoya:Molecular-weight-dependent, Anionic-substrate-preferential Transport of beta-Lactam Antibiotics via Multidrug Resistance-associated Protein 4.Drug Metab Pharmacokinet.26:602-11 (2011)
  7. S. Ito, S. Ohtsuki, Y. Katsukura, M. Funaki, Y. Koitabashi, A. Sugino, S. Murata, T. Terasaki:Atrial natriuretic peptide is eliminated from the brain by natriuretic peptide receptor-C-mediated brain-to-blood efflux transport at the blood-brain barrier.J Cereb Blood Flow Metab.31:457-66 (2011)
  8. H. Kawakami, S. Ohtsuki, J. Kamiie, T. Suzuki, T. Abe, T. Terasaki:Simultaneous absolute quantification of 11 cytochrome P450 isoforms in human liver microsomes by liquid chromatography tandem mass spectrometry with in silico target peptide selection.J Pharm Sci.100:341-52 (2011)
  9. F. Shimizu, Y. Sano, M.A. Abe, T. Maeda, S. Ohtsuki, T. Terasaki, T. Kanda:Peripheral nerve pericytes modify the blood-nerve barrier function and tight junctional molecules through the secretion of various soluble factors.J Cell Physiol.226:255-66 (2011)
  10. S. Ito, S. Ohtsuki, Y. Nezu, Y. Koitabashi, S. Murata, T. Terasaki:1a,25-Dihydroxyvitamin D3 enhances cerebral clearance of human amyloid-b peptide(1-40) from mouse brain across the blood-brain barrier.Fluids Barriers CNS.8:20 (2011)
  11. K. Ito, Y. Uchida, S. Ohtsuki, S. Aizawa, H. Kawakami, Y. Katsukura, J. Kamiie, T. Terasaki:Quantitative membrane protein expression at the blood-brain barrier of adult and younger cynomolgus monkeys.J Pharm Sci.100:3939-50 (2011)
  12. T. Okura, S. Kato, Y. Takano, T. Sato, A. Yamashita, R. Morimoto, S. Ohtsuki, T. Terasaki, Y. Deguchi:Functional characterization of rat plasma membrane monoamine transporter in the blood-brain and blood-cerebrospinal fluid barriers.J Pharm Sci.100:3924-38 (2011)
  13. A. Sakamoto, T. Matsumaru, N. Ishiguro, O. Schaefer, S. Ohtsuki, T. Inoue, H. Kawakami, T. Terasaki:Reliability and robustness of simultaneous absolute quantification of drug transporters, cytochrome P450 enzymes, and Udp-glucuronosyltransferases in human liver tissue by multiplexed MRM/selected reaction monitoring mode tandem mass spectrometry with nano-liquid chromatography.J Pharm Sci.100:4037-43 (2011)
  14. N.Y. Lee, Y. Sai, E. Nakashima, S. Ohtsuki, Y.S. Kang:6-Mercaptopurine transport by equilibrative nucleoside transporters in conditionally immortalized rat syncytiotrophoblast cell lines TR-TBTs.J Pharm Sci.100:3773-82 (2011)
Review Articles
  1. S. Ohtsuki, Y. Uchida, Y. Kubo, T. Terasaki:Quantitative targeted absolute proteomics-based ADME research as a new path to drug discovery and development: Methodology, advantages, strategy, and prospects.J Pharm Sci.100:3547-59 (2011)
総説
  1. 内田康雄、大槻純男、寺崎哲也：Pharmacoproteomics (PPx)に基づく血液脳関門輸送機能の解明、遺伝子医学MOOK19、148-154 (2011)
  2. 大槻純男：脳関門輸送機構を利用した神経変性疾患に対する新たな治療戦略の構築、インテリジェントコスモス学術振興財団紀要、15:13-16 (2011)
  3. 元井冬彦、川口桂、大峰健、大槻純男、大塚英郎、力山敏樹、片寄友、江川新一、寺崎哲也、海野倫明：膵がん個別化医療-分子生物学形跡を中心に-、外科、73:1073-1078 (2011)
  4. 立川正憲、内田康雄、大槻純男、寺崎哲也：定量的絶対標的プロテオミクスに基づくヒト血液脳関門におけるトランスポーターの発現と生理機能、脳21、14:42-48 (2011)